Changes in Lolium perenne L. rhizosphere microbiome during phytoremediation of Cd- and Hg-contaminated soils

• DOI: 10.1007/s11356-023-25501-y
• Publication Date: 2023-02-13T19:04:35Z
• Authors: Juan F. Saldarriaga; Julián E. López; Laura Díaz-García; Carolina Montoya-...
• Keywords: 2. Zero hunger; 0301 basic medicine; Mercury; 15. Life on land; 6. Clean water; Soil; 03 medical and health sciences; Biodegradation, Environmental; Metals; Mycorrhizae; Rhizosphere; Lolium; Humans; Soil Pollutants; Research Article; Cadmium

Abstract The contamination of soil and water by metals such as mercury (Hg) cadmium (Cd) has been increasing in recent years, because anthropogenic activities mining agriculture, respectively. In this work, the changes rhizosphere microbiome Lolium perenne L. during phytoremediation soils contaminated with Hg Cd were evaluated. For this, two types sampled, one inoculated mycorrhizae without. Cd, seeds sown harvested after 30 days. To assess microbiome, DNA isolation tests performed, for which samples subjected to two-step PCR amplification specific 16S rDNA V3-V4 primers (337F 805R). With mycorrhizae, had found absorption processes a new distribution. While respect microorganisms, families Enterobacteriaceae have shown biosorption efflux effects on Cd. Mycorrhizae then improve efficiency removal allow plant better distribute absorbed concentrations. Overall, is species high potential Cd- Hg-contaminated tropics. Inoculation modifies mechanisms composition microorganisms rhizosphere. Mycorrhizal inoculation associated increased tolerance Hg. Microorganism-assisted an appropriate alternative .