Shifts in structure and dynamics of the soil microbiome in biofuel/fuel blend–affected areas triggered by different bioremediation treatments

• DOI: 10.1007/s11356-024-33304-y
• Publication Date: 2024-04-30T06:02:11Z
• Authors: Kelly Hidalgo-Mar...; Admir José Giachini; Marcio Schneider; Adriana Soriano; Marcus Paulus Baessa; Luiz Fernando Mar...; Valéria Maia de O...
• Keywords: Biostimulation; Soil microbiology

Abstract The use of biofuels has grown in the last decades, due to environmental impacts of fossil fuels use. Elucidating structure, diversity, species interactions and assembly mechanisms of microbiomes is crucial for understanding the influence of environmental disturbances. However, little is known about how contamination with biofuel/petrofuel blends alters the soil microbiome. This study aimed to characterize the soil microbiome of four long term field experimental areas that received controlled releases of E10, E25 or B20 and were submitted to different bioremediation treatments, using 16S rRNA gene amplicon high throughput sequencing. Results indicated that the soil microbiome of biodiesel-areas is more diverse, resilient, and complex. It was also observed that in soils with low diversity and richness, the impact of bioremediation treatments on the microbiomes was higher. The network analysis showed that after applying the bioremediation treatment, the proportion of generalist taxa increased, suggesting a more connected and dynamic assembly. All abundant keystone taxa are well-known degraders, suggesting that the abundant species are core targets for biostimulation in soil remediation. Overall, these findings extend our knowledge of the soil microbiome response triggered by pollution stress and bioremediation treatments, paving the way for future rationalized and efficient pollutant mitigation strategies.