Identification of a suitable endogenous control gene in porcine blastocysts for use in quantitative PCR analysis of microRNAs

• DOI: 10.1007/s11427-012-4289-8
• Publication Date: 2012-03-14T02:16:31Z
• Authors: Jun Li; HengXi Wei; Yan Li; QiuYan Li; Ning Li
• Keywords: Reference Genes; TaqMan

To obtain reliable results in quantitative PCR (qPCR) reactions, an endogenous control (EC) gene is needed to correct for systematic variations. In this study, a TaqMan low density array was used quantify the expression levels of microRNA (miRNA) genes vivo fertilized, vitro parthenogenetic and somatic cell nuclear transfer blastocysts. The aim identify suitable EC qPCR analysis miRNAs porcine showed that thirty-six were commonly expressed four kinds embryos eleven similar different embryo types (P-value>0.05). These 11 selected as candidate further and, these, miR-16 identified most stable by GeNorm (a tool based on pair-wise comparison model calculates internal stability measure determines pair genes) NormFinder (an excel plug-in uses ANOVA-based estimate intra- inter-group variation indicate single gene) programs. addition, number normalization method validated use future