Non-integrating lentiviral vectors based on the minimal S/MAR sequence retain transgene expression in dividing cells

• DOI: 10.1007/s11427-016-0067-0
• Publication Date: 2016-09-10T04:42:09Z
• Authors: Zhen Xu; Feng Chen; Lingling Zhang; Jing Lu; Peng Xu; Guang Liu; Xuemin Xie; Wenli Mu; Yajun Wang; Depei Liu
• Keywords: Insertional mutagenesis; Scaffold/matrix attachment region; Sequence (biology)

Safe and efficient gene transfer systems are the basis of therapy applications. Non-integrating lentiviral (NIL) vectors among most promising candidates for tools, because they exhibit high efficiency in both dividing non-dividing cells do not present a risk insertional mutagenesis. However, non-integrating cannot introduce stable exogenous expression to cells, thereby limiting their application. Here, we report design vector that contains minimal scaffold/matrix attachment region (S/MAR) sequence (SNIL), this SNIL is able retain episomal transgene cells. Using vectors, detected eGFP 61 days SNIL-transduced CHO either with selection or not. In NIL group without S/MAR sequence, however, transduced died under transient vectors. Furthermore, Southern blot assays demonstrated were retained extrachromosomally conclusion, which indicates have potential use as tool.