Nerve growth factor inhibits Gd3+-sensitive calcium influx and reduces chemical anoxic neuronal death

• DOI: 10.1007/s11596-008-0402-4
• Publication Date: 2010-06-22T17:48:51Z
• Authors: Hui Jiang; Shunlian Tian; Yan Zeng; Jing Shi
• Keywords: Neurons; 0301 basic medicine; Cell Death; Glutamic Acid; Gadolinium; Hippocampus; Cell Hypoxia; Rats; Rats, Sprague-Dawley; 03 medical and health sciences; Neuroprotective Agents; Animals, Newborn; Nerve Degeneration; Nerve Growth Factor; Animals; Calcium; Excitatory Amino Acid Antagonists; Cells, Cultured

To investigate whether glutamate and voltage-gated calcium channels-independent calcium influx exists during acute anoxic neuronal damage and its possible relationship to neuronal protective function of NGF. In in vitro model of acute anoxia, hippocampal cultures from newborn rats were exposed to 3 mmol/L KCN. Changes of intracellular Ca(2+) concentration ([Ca(2+)](i)) were monitored by con-focal imaging and cell viability was assayed by PI and cFDA staining. The results showed that after treatment with primary hippocampal cultures with 3 mmol/L KCN for 15 min, [Ca(2+)](i) was significantly increased 6.27-fold compared to pre-anoxia level and 73.3% of the cells died. When combination of 20 micromol/L MK-801 (glutamate receptor antagonist), 40 micromol/L CNQX (AMPA receptor antagonist) and 5 micromol/L nimodipine (voltage-gated calcium channel antagonist) (hereafter denoted as MCN) were administrated to hippocampal cultures, levels of [Ca(2+)](i) and cell death rate induced by KCN were partially reduced by 35.9% and 47.5% respectively. However, Gd(3+) (10 micromol/L) almost completely blocked KCN-mediated [Ca(2+)](i) elevation by 81.9% and reduced neuronal death by 88.8% in the presence of MCN. It is noteworthy that NGF, used in combination with MCN, inhibited KCN-induced [Ca(2+)](i) increase by 77.4% and reduced cell death by 87.1%. Only PLC inhibitor U73122 (10 micromol/L) abolished NGF effects. It is concluded that Gd(3+)-sensitive calcium influx, which is NMDA (glutamate receptor) and voltage-gated calcium channels-independent, is responsible for acute anoxic neuronal death. NGF can inhibit Gd(3+)-sensitive calcium influx and reduce anoxic neuronal death through activating PLC pathway.