Static magnetic field enhances synthesis and secretion of membrane-derived microvesicles (MVs) rich in VEGF and BMP-2 in equine adipose-derived stromal cells (EqASCs)—a new approach in veterinary regenerative medicine

• DOI: 10.1007/s11626-014-9828-0
• Publication Date: 2014-12-01T11:47:51Z
• Authors: Monika Marędziak; Krzysztof Marycz; Daniel Lewandowski; Anna Siudzińska; Agnieszka Śmieszek
• Keywords: Regenerative Medicine

The aim of this work study was to evaluate the cytophysiological activity equine adipose-derived stem cells (ASCs) cultured under conditions static magnetic field. Investigated were exposed a field (MF) with intensity 0.5 T. In order investigate effects on cell signaling, localization and density content microvesicles (MVs) as well morphology, ultrastructure, proliferation rate ASCs evaluated. Results showed that potential mesenchymal accelerated when applied. Resazurin-based assay indicated in reached population doubling time earlier colony-forming higher conditions. Morphological ultrastructural examination exposure did not cause any significant changes morphology whereas polarity observed examinations. Exposition MF resulted considerable increase number secreted MVs—we have clearly differences between numbers MVs shed from comparison control culture rich growth factors. Microvesicles derived condition might be utilized cell-based treatment musculoskeletal disorders tendon injuries.