Micropropagation of Heracleum candicans wall: A rare medicinal herb

• DOI: 10.1007/s11627-999-0014-0
• Publication Date: 2007-05-18T07:29:07Z
• Authors: A. K. Wakhlu; R. K. Sharma
• Keywords: 0303 health sciences; 03 medical and health sciences; 3. Good health

A protocol was developed for micropropagation of Heracleum candicans Wall. by axillary shoot proliferation. Maximum shoot proliferation was obtained on Murashige and Skoog medium supplemented with 0.5 mg (2.2 µM) 6-benzyladenine per 1 and 0.1 mg (0.4 µM) 1-naphthaleneacetic acid per 1. Regenerated shoots were rooted on MS medium fortified with 1 mg (4.9 µM) indole-3-butyric acid per 1. Complete plants were transferred to soil and all of these plants were morphologically and cytologically identical to the mother plant.