Electro-Immobilization of Acetylcholinesterase Using Polydopamine for Carbaryl Microsensor

• DOI: 10.1007/s11664-017-5880-3
• Publication Date: 2017-10-26T09:46:21Z
• Authors: Trung B. Ha; Huyen T. Le; Ha H. Cao; Nguyen Thanh Binh; Huy L. Nguyen; Le Hai Dang; Quan P. Do; Dzung T. Nguyen; Tran Dai Lam; Vân-Anh Nguyen
• Keywords: Carbaryl; Solid-state physics

A simple and sensitive electrochemical acetylcholinesterase (AChE) biosensor for determination of carbaryl, one of the most commonly used carbamate pesticides, is described. The AChE enzyme was successfully entrapped by a polydopamine–graphene composite on polypyrrole nanowires that modified interdigitated planar platinum-film microelectrodes . The influence of different parameters on the operation of the biosensor was also studied. The selected parameters for the biosensor performance in detecting carbaryl were as follows: applied potential + 0.7 V, pH 7.4 at 25°C. The inhibition of carbaryl was proportional to its concentrations ranging from 0.05 to 1.5 μg/mL with the detection limit of 0.008 μg/mL using chronoamperometry. This study provides a promising approach in fabrication of sensitive biosensors for the analysis of carbamate pesticides as well as other hazardous compounds.