The Effect of One Anastomosis Gastric Bypass on Branched-Chain Fatty Acid and Branched-Chain Amino Acid Metabolism in Subjects with Morbid Obesity

• DOI: 10.1007/s11695-019-04157-z
• Publication Date: 2019-08-22T05:02:37Z
• Authors: Alicja Pakiet; Maciej Wilczynski; Olga Rostkowska; Justyna Korczynska; Patrycja Jabłonska; Lukasz Kaska; Monika Proczko-St...; Ewa Sobczak; Piotr Stepnowski; Faidon Magkos; Tomasz Sledzinski; Adriana Mika
• Keywords: Adult; Male; 0301 basic medicine; bariatric surgery; Gastric Bypass; Branched-chain fatty acids; Morbid obesity; 03 medical and health sciences; insulin resistance; Weight Loss; Faculty of Science; Humans; /dk/atira/pure/core/keywords/TheFacultyOfScience; branched-chain amino acids; Bariatric surgery; 2. Zero hunger; Fatty Acids; Insulin resistance; Middle Aged; Branched-chain amino acids; morbid obesity; Obesity, Morbid; 3. Good health; Treatment Outcome; Adipose Tissue; /dk/atira/pure/core/keywords/TheFacultyOfScience; name=Faculty of Science; branched-chain fatty acids; Female; Poland; Insulin Resistance; Amino Acids, Branched-Chain; Follow-Up Studies

Subjects with morbid obesity have low levels of serum branched-chain fatty acids (BCFAs), which correlate inversely with insulin resistance, hypertriglyceridemia, and inflammation. Recent evidence suggests BCFAs are produced during branched-chain amino acid (BCAA) catabolism in human adipose tissue. Elevated concentrations of BCAAs are associated with insulin resistance.In this single-center study, we evaluated the effect of one anastomosis gastric bypass (OAGB) on circulating BCFA and BCAA. Moreover, we determined the expression of genes involved in BCAA catabolism in adipose tissue of patients with obesity and lean controls.Fasting levels of BCFAs and BCAAs were determined by gas and liquid chromatography, respectively, coupled with mass spectrometry, in 50 patients with morbid obesity before and 6-9 months after surgery, and in 32 lean controls. Visceral and subcutaneous adipose tissue (VAT and SAT, respectively) biopsies were collected at baseline to determine mRNA levels for enzymes involved in BCAA catabolism.Before surgery, patients with obesity had lower BCFAs and greater BCAAs than control subjects. OAGB increased BCFA and decreased BCAA levels. Insulin resistance (assessed by HOMA) correlated inversely with BCFAs and positively with BCAAs. Expression of genes involved in BCAA catabolism in VAT (but not SAT) was lower in patients with obesity than in lean controls.OAGB-induced weight loss increases circulating BCFAs and decreases circulating BCAAs in patients with morbid obesity, perhaps by altering BCAA catabolism in VAT. We speculate that this shift may be related to the improvement in insulin sensitivity after surgery.