Effects of macro elements and nitrogen source on biomass accumulation and bacoside A production from adventitious shoot cultures of Bacopa monnieri (L.)

• DOI: 10.1007/s11738-010-0675-7
• Publication Date: 2010-12-23T11:01:14Z
• Authors: Poornananda Madha...; Shirugumbi Hanama...; Hosakatte Niranja...
• Keywords: Bacopa monnieri; Dry weight

The present work deals with optimization of adventitious shoot culture of Bacopa monnieri for the production of biomass and bacoside A and has investigated the effects of macro elements (NH4NO3, KNO3, CaCl2, MgSO4 and KH2PO4) and nitrogen source [NH4+/NO3−] of Murashige and Skoog (Physiol Plant 15:473–497, 1962) medium (MS) on accumulation of biomass and bacoside A content. Optimum number of adventitious shoots (99.33 shoots explant−1), fresh weight (1.841 g) and dry weight (0.150 g) were obtained in the medium with 2.0× strength of NH4NO3. The highest production of bacoside A content was also recorded in the medium of 2.0× NH4NO3, which produced 17.935 mg g−1 DW. The number of adventitious shoot biomass and bacoside A content were optimum when the NO3− concentration was higher than that of NH4+. Maximum number of shoots (70.00 shoots explant−1), biomass (fresh weight 1.137 g and dry weight 0.080 g) and also bacoside A content (27.106 mg g−1 DW) were obtained at NH4+/NO3− ratio of 14.38/37.60 mM. Overall, MS medium supplemented with 2.0× NH4NO3 is recommended for most efficient bacoside A production.