Response of barley seedlings to water deficit and enhanced UV-B irradiation acting alone and in combination

• DOI: 10.1007/s11738-011-0814-9
• Publication Date: 2011-07-26T06:23:42Z
• Authors: Hanna Bandurska; Małgorzata Pietro...; Małgorzata Cieślak
• Keywords: Malondialdehyde; Phenylpropanoid; Plant Physiology

Responses of barley seedlings to water deficit (WD) induced by polyethylene glycol (PEG 6000) and ultraviolet (UV-B; 280–320 nm) radiation their interaction (UV-B + WD) were examined. A decrease in dry matter yield content leaves roots was observed following application WD UV-B WD, while no changes found after treating plants with UV-B. Proline increased under conditions WD. In contrast, treatment had effect on the accumulation proline plants. Changes root showed a varied response: an increase level this amino acid, as well suppressed content. The lipid peroxidation product malondialdehyde (MDA) stresses. Root MDA WD-stressed plants, but it decreased case combined both applied stress factors operated variable manner phenylpropanoid metabolism. Phenylalanine ammonia-lyase (PAL) activity stimulated exposure stresses, did not affect its activity. On other hand, enhanced 4:coumarate-CoA ligase (4CL) enhancement positively correlated anthocyanins flavonols. However, reduced positive these compounds 4CL. Surprisingly, flavonols detected examined despite 4CL results suggest that UV-B-induced activation anthocyanin is beneficial for response factor. WD-induced reduction contents flavonol might be cause membrane damage UV-B- addition, conversely what could expected, perceived water-stressed roots, which exhibited exposed