Fatty Acid Profile of the Initial Oral Biofilm (Pellicle): an In‐Situ Study

• DOI: 10.1007/s11745-013-3822-2
• Publication Date: 2013-08-02T07:40:27Z
• Authors: Marco Reich; Klaus Kümmerer; Ali Al‐Ahmad; Christian Hannig
• Keywords: Lipidology

The first step of bioadhesion on dental surfaces is the formation acquired pellicle. This mainly acellular layer formed instantaneously all solid exposed to oral fluids. It composed proteins, glycoproteins and lipids. However, information lipid composition sparse. aim present study was evaluate fatty acid (FA) profile in-situ pellicle for time. Furthermore, impact rinses with safflower oil pellicle's FA investigated. Pellicles were in situ bovine enamel slabs mounted individual upper jaw splints. splints carried by ten subjects over durations 3-240 min. After comprehensive sample preparation, gas chromatography coupled electron ionization mass spectrometry (GC-EI/MS) used order characterize qualitatively quantitatively a wide range (C12-C24). relative profiles samples gained from different remarkably similar, whereas amount showed significant interindividual variability. An increase observed application resulted an accumulation its specific Pellicle highly selective process that does not correlate directly salivary composition, as shown FA.