Purification of steryl esters from soybean oil deodorizer distillate

• DOI: 10.1007/s11746-003-0700-6
• Publication Date: 2006-11-22T11:18:16Z
• Authors: Yoshinori Hirota; Toshihiro Nagao; Yomi Watanabe; Masaharu Suenaga; Seiichi Nakai; Aktohiro Kitano; Akio Sugihara; Yuji Shimada
• Keywords: Glyceride; Soybean oil

Abstract Soybean oil deodorizer distillate (SODD) contains steryl esters in addition to tocopherols and sterols. Tocopherols sterols have been industrially purified from SODD but no purification process for has developed. was efficiently separated low b.p. substances (including sterols) high 11.2 wt% DAG, 32.1 TAG, 45.4 esters) by molecular distillation. The fraction is referred as soybean ester concentrate (SODDSEC). We attempted purify after a lipase‐catalyzed hydrolysis of acylglycerols SODDSEC. Screening available lipases indicated that Candida rugosa lipase most effective. Based on the study several factors affecting hydrolysis, reaction conditions were determined follows: ratio SODDSEC/water, 1∶1 (w/w); amount, 15 U/g mixture; temperature, 30°C. When SODDSEC agitated 24 h under these conditions, almost completely hydrolyzed content did not change. However, with mixture oleate/trilinolein (1∶1, w/w) about 20% constituent FA exchanged acylglycerols. Steryl layer obtained treatment successfully distillation (purity, 97.3%; recovery, 87.7%).