Purification of tocopherols and phytosterols by a two‐step in situ enzymatic reaction

• DOI: 10.1007/s11746-004-0904-9
• Publication Date: 2006-11-22T12:08:35Z
• Authors: Yomi Watanabe; Toshihiro Nagao; Yoshinori Hirota; Motohiro Kitano; Yuji Shimada
• Keywords: Candida rugosa; Glyceride; Phytosterol

Abstract The purification of tocopherols and phytosterols (referred to as sterols) from soybean oil deodorizer distillate (SODD) was attempted. Tocopherols sterols in the SODD were first recovered by short‐path distillation, which named sODD tocopherol/sterol concentrate (SODDTSC). SODD‐TSC contained MAG, DAG, FFA, unidentified hydrocarbons addition two substances interest. It then treated with Candida rugosa lipase convert FA steryl esters, acylglycerols FFA FAME. Methanol (MeOH), however, inhibited esterification sterols. Hence, a two‐step situ reaction conducted: SODDTSC stirred 20 wt% water 200 U/g mixture C. at 30°C, 2 moles MeOH per mole added after 16h. treatment for 40 h total achieved 80% conversion initial complete hydrolysis acylglycerols, 78% decrease content methyl esterification. did not change throughout process. To enhance degree esterification, products, esters FAME, removed resulting fraction containing tocopherols, sterols, again. Distillation purified 76.4% (recovery, 89.6%) 97.2% 86.3%).