Microscale recovery of total lipids from fish tissue by accelerated solvent extraction

• DOI: 10.1007/s11746-004-0988-2
• Publication Date: 2006-11-28T14:29:04Z
• Authors: Eric D. Dodds; Mark R. McCoy; Adeline Geldenhuys; Lorrie D. Rea; John M. Kennish
• Keywords: Microscale chemistry; Accelerated solvent extraction; Repeatability; Sample Preparation

Abstract A number of techniques are available for the extraction lipids from a variety tissues; however, conventional methods characteristically labor intensive, typically involve large volumes toxic solvents, and usually require at least 1 g tissue. With availability accelerated solvent (ASE) technology, opportunity exists to modify classical lipid such that automated high‐pressure, high‐temperature extractions may be performed with use far smaller costly harmsul solvents. Moreover, high efficiency attainable by ASE suggests significantly less tissue would required than is routinely used. This paper describes adaptation previously developed systems toward purpose extracting total 100 mg fish The efficacy three representative tissues, including standard reference material, was explored using gravimetry FA analysis GC. TG used as surrogate monitor overall method performance. findings herein demonstrate microscale represents an effective efficient alternative traditional based on quantity composition extracted lipid, recovery, precision.