MiR-1a-3p Inhibits Apoptosis in Fluoride-exposed LS8 Cells by Targeting Map3k1

• DOI: 10.1007/s12011-023-03869-9
• Publication Date: 2023-10-02T09:01:55Z
• Authors: Ting Chen; Yu Gu; Guo-Hui Bai; Xia Liu; Bin Chen; Qin Fan; Jian-Guo Liu; Yuan Tian
• Keywords: KEGG

Dental fluorosis is a common chemical disease. It currently unclear how occurs at the molecular level. We used miRNA-seq to look differences between miRNAs in cell line of ameloblasts LS8 that had been treated with 3.2 mmol/L NaF. also performed gene ontology (GO) and Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment analyses. miR-1a-3p levels were significantly lower mouse cells NaF, miR-1a-3p-targeted genes enriched MAPK pathway. divided into four groups: control, NaF+miR-1a-3p mimics, mimics normal control groups. Cellular morphology was observed by an inverted microscope, proliferation activity assessed Cell Counting Kit-8 (CCK-8). Using real-time quantitative polymerase chain reaction (RT-qPCR), transcription Map3k1 detected. The expressions Bax, Bcl-2, Map3k1, p38MAPK, ERK1/2, p-p38MAPK, p-ERK1/2 measured Western blot. After bioinformatics analysis, we luciferase reporter assay (LRA) validate target miR-1a-3p, showing could inhibit apoptosis while increasing fluoride-exposed cells. Generally, might directly reduce signal activation, promote phosphorylation. Thus, our findings revealed interaction its decrease