Optimization of methods for intrasplenic administration of human amniotic epithelial cells in order to perform safe and effective cell-based therapy for liver diseases

• DOI: 10.1007/s12015-024-10735-1
• Publication Date: 2024-05-21T02:01:44Z
• Authors: Piotr Czekaj; Mateusz Król; Emanuel Kolanko; Patrycja Wieczorek; Edyta Bogunia; Mateusz Hermyt; Aniela Grajoszek; Agnieszka Prusek
• Keywords: Amniotic epithelial cells; Cell therapy

Abstract In animal experimental models the administration of stem cells into spleen should ensure high effectiveness their implantation in liver due to a direct vascular connection between two organs. The aim this study was update methods intrasplenic cell transplantation using human amniotic epithelial (hAECs) which are promising treatment diseases. BALB/c mice were administered intrasplenically with 0.5, 1, and 2 million hAECs by bolus injection (400 µl/min) via subcutaneous splenic port fast (20 μl/min) slow (10 infusion. prepared translocating skin pocket. spleen, liver, lungs collected at 3 h, 6 24 h after cells. distribution hAECs, histopathological changes organs, complete blood count, biochemical markers damage assessed. It has been shown that method affects degree damage. largest number showing significant observed lowest through port. Liver increased cells, which, paradoxically, resulted colonization efficiency. concluded 1 × 10 infusion reduces incidence complications expense slight decrease transplanted liver.