IL-1α-induced microvascular endothelial cells promote neutrophil killing by increasing MMP-9 concentration and lysozyme activity

• DOI: 10.1007/s12026-015-8731-4
• Publication Date: 2015-12-11T06:55:25Z
• Authors: Xiaoye Liu; Hong Dong; Mingming Wang; Ying Gao; Tao Zhang; Ge Hu; Huiqing Duan; Xiang Mu
• Keywords: Cytotoxicity, Immunologic; Lipopolysaccharides; 0303 health sciences; Neutrophils; Transendothelial and Transepithelial Migration; Endothelial Cells; Rats, Inbred Strains; Bacterial Infections; Rats; 3. Good health; 03 medical and health sciences; Gene Expression Regulation; Matrix Metalloproteinase 9; Interleukin-1alpha; Host-Pathogen Interactions; Animals; Muramidase; Cells, Cultured

The recruitment of neutrophils by endothelial cells during infection has been extensively studied, but little is known about the regulation of neutrophils activity by endothelial cells. To examine the role of microvascular endothelial cells in neutrophil killing, we established a transmigration model using rat intestinal microvascular endothelial cells (RIMVECs) and measured the extracellular and intracellular killing of Escherichia coli, Lactobacillus acidophilus, and Staphylococcus aureus by transendothelial neutrophils. We observed that blood neutrophils engulfed bacteria but did not kill them, and lipopolysaccharide- or hemolysin-injured RIMVECs inhibited the extracellular and intracellular bactericidal activity of transendothelial neutrophils. In comparison, interleukin-1α-induced RIMVECs promoted the extracellular and intracellular killing activity of transendothelial neutrophils and significantly increased MMP-9 concentration and lysozyme activity in transendothelial neutrophils (p < 0.01 and p < 0.001, respectively). Our results demonstrated that activation of endothelial cells enhanced bactericidal activity of transendothelial neutrophils and bacterial toxin damage of endothelial cells led to reduction in bactericidal activity of transendothelial neutrophils. These findings offered new insight into the role of endothelial cells in the bactericidal activity of neutrophils.