Influence of miR-30b regulating humoral immune response by genetic difference

• DOI: 10.1007/s12026-015-8736-z
• Publication Date: 2015-11-21T00:19:41Z
• Authors: Zhi-Qing Duan; Jian-Dong Shi; Mei-Ni Wu; Ning-Zhu Hu; Yun-Zhang Hu
• Keywords: Immunosuppression Therapy; Lipopolysaccharides; 0301 basic medicine; B-Lymphocytes; Polymorphism, Genetic; Immunity, Humoral; 3. Good health; Mice; MicroRNAs; 03 medical and health sciences; Basic-Leucine Zipper Transcription Factors; Gene Expression Regulation; Immunoglobulin M; Receptors, Aryl Hydrocarbon; Cell Line, Tumor; Animals; Humans; Kynurenine

Investigation of genetic difference will be beneficial to researchers to understand the origins and nature of diseases. Previous studies have revealed that L-kynurenine (L-Kyn) level was changed significantly in patient with cancer and that miR-30b play different role in tumor cells and immune cells. Moreover, it has been also conformed that miR-30b involved in the process of L-Kyn-mediated suppression of humoral immune responses induced by lipopolysaccharide (LPS) in human normal B cells separated from volunteers' peripheral blood. Nevertheless, the miR-30b role regulating humoral immune response in B lymphoma cells has been still unclear due to the genetic difference between normal cells and tumor cells. The current study demonstrated that the selected concentration of L-Kyn (100, 1000 μM) significantly reduced the immunoglobulin M secretion induced by LPS when compared with the control group in B lymphoma, CH12.LX, and BCL-1 cells, which had, at least, incomplete dependence on Aryl hydrocarbon receptor, the receptor of L-Kyn. In addition, although L-Kyn (100 μM) significantly attenuated the expression of miR-30b in BCL-1 cells rather than in CH12.LX cells, no significant differences in the strength of L-Kyn-mediated suppression of humoral immune responses induced by LPS were detected by enzyme-linked immunosorbent assay between the LPS (10 μg/ml) + L-Kyn (100 μM) group and the LPS (10 μg/ml) + L-Kyn (100 μM) + miR-30b mimics/miR-30b inhibitor group in CH12.LX and BCL-1 cells, respectively. Further data also showed that mouse Bach2 mRNA was a novel target of miR-30b. These results suggest that genetic difference among cells has a great influence on the miR-30b role in the process of L-Kyn-mediated suppression of humoral immune responses induced by LPS.