High Serum DNA and RNA Oxidative Damage in Non-surviving Patients with Spontaneous Intracerebral Hemorrhage

• DOI: 10.1007/s12028-019-00864-8
• Publication Date: 2019-10-30T17:50:16Z
• Authors: Leonardo Lorente; María M. Martín; Agustín F. Gonzál...; Antonia Pérez-Cejas; Pedro Abreu-González; Rafael Sabatel; Luis Ramos; Mónica Argueso; Juan J. Cáceres; Jordi Solé-Violán; Alejandro Jiménez; Victor García-Marín
• Keywords: Male; 0301 basic medicine; Guanine; Guanosine; DNA; Middle Aged; Prognosis; 3. Good health; Oxidative Stress; 03 medical and health sciences; 8-Hydroxy-2'-Deoxyguanosine; Humans; RNA; Female; Glasgow Coma Scale; Prospective Studies; Mortality; Aged; Cerebral Hemorrhage; DNA Damage

One study found higher leukocytes 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels in patients with spontaneous intracerebral hemorrhage (ICH) than in healthy subjects due to the oxidation of guanosine from deoxyribonucleic acid (DNA). The objective of this study was to determine whether there is an association between oxidative damage of serum DNA and ribonucleic acid (RNA) and mortality in patients with ICH.In this observational and prospective study, patients with severe supratentorial ICH (defined as Glasgow Coma Scale < 9) were included from six Intensive Care Units of Spanish hospitals. At the time of severe ICH diagnosis, concentrations in serum of malondialdehyde (as lipid peroxidation biomarker) and of the three oxidized guanine species (OGS) (8-hydroxyguanosine from RNA, 8-hydroxyguanine from DNA or RNA, and 8-OHdG from DNA) were determined. Thirty-day mortality was considered the end-point study.Serum levels of OGS (p < 0.001) and malondialdehyde (p = 0.002) were higher in non-surviving (n = 46) than in surviving patients (n = 54). There was an association of serum OGS levels with serum malondialdehyde levels (rho = 0.36; p = 0.001) and 30-day mortality (OR = 1.568; 95% CI 1.183-2.078; p = 0.002).The novel and most important finding of our study was that serum OGS levels in ICH patients are associated with mortality.