MiR-615 Agomir Encapsulated in Pluronic F-127 Alleviates Neuron Damage and Facilitates Function Recovery After Brachial Plexus Avulsion

• DOI: 10.1007/s12031-021-01916-5
• Publication Date: 2021-09-26T23:19:12Z
• Authors: Kangzhen Chen; Lu Ding; Hua Shui; Yinru Liang; Xiaomin Zhang; Tao Wang; Linke Li; Shuxian Liu; Hongfu Wu
• Keywords: Neurochemistry

Brachial plexus avulsion (BPA) is a devastating traumatic peripheral nerve injury complicated with paralysis of the upper extremity. We previously reported that leucine-rich repeat and immunoglobulin-like domain-containing NOGO receptor-interacting protein 1 (LINGO-1) has potent role in inhibiting neuron survival axonal regeneration after central nervous system (CNS) damage miR-615 potential microRNA (miRNA) negatively regulated LINGO-1. However, effect BPA remains to be elucidated. Accumulating evidence indicates pluronic F-127 (PF-127) hydrogel could serve as promising vehicle for miRNA encapsulation. Thus, further explore hydrogel-miR-615 BPA-reimplantation, present study established rat model injected agomir encapsulated by PF-127 into reimplantation site using microsyringe. In this study, results indicated effectively alleviated motoneuron loss LINGO-1 inhibition, promoted musculocutaneous myelination, reduced astrocytes activation, angiogenesis attenuated amyotrophy, leading improved motor functional rehabilitation conclusion, our findings demonstrate miR-615-loaded may represent novel therapeutic strategy treatment.