RNAi-mediated downregulation of NOB1 suppresses the growth and colony-formation ability of human ovarian cancer cells

• DOI: 10.1007/s12032-010-9808-5
• Publication Date: 2011-02-01T15:41:23Z
• Authors: Yang Lin; Shuai Peng; Hansong Yu; Hong Teng; Manhua Cui
• Keywords: Ovarian Neoplasms; 0303 health sciences; Reverse Transcriptase Polymerase Chain Reaction; Blotting, Western; Down-Regulation; Nuclear Proteins; RNA-Binding Proteins; Cell Cycle Checkpoints; Flow Cytometry; Real-Time Polymerase Chain Reaction; Transfection; 3. Good health; 03 medical and health sciences; Cell Line, Tumor; Gene Knockdown Techniques; Humans; Female; RNA Interference; RNA, Small Interfering; Cell Proliferation

Nin one binding protein (NOB1p), encoded by the NOB1 gene, is a crucial molecule in the maturation of the 20S proteasome and protein degradation. The present study evaluates whether NOB1 is an appropriate molecular target for cancer gene therapy. In two ovarian cancer cell lines, SKOV3 and HEY, NOB1 expression was knocked down by a lentiviral short hairpin RNA (shRNA) delivery system. The RNA interference (RNAi)-mediated the downregulation of NOB1 expression markedly reduced the proliferative and colony-formation ability of ovarian cancer cells. Additionally, NOB1 shRNA-expressing lentivirus-treated ovarian cancer cells tended to arrest in the G0/G1 phase. These results suggested that NOB1 may act as an oncogenic factor in ovarian cancer and could be a potential molecular target for ovarian cancer gene therapy.