Genome-Wide DNA Methylation Patterns Analysis of Noncoding RNAs in Temporal Lobe Epilepsy Patients

• DOI: 10.1007/s12035-016-0353-x
• Publication Date: 2017-01-05T06:48:56Z
• Authors: Wenbiao Xiao; Yuze Cao; Hongyu Long; Zhaohui Luo; Shuyu Li; Na Deng; Jianjian Wang; Xiaoyan Lu; Tianfeng Wang; Shangwei Ning; Lihua Wang; Bo Xiao
• Keywords: Adult; Male; 0301 basic medicine; RNA, Untranslated; Genome, Human; Molecular Sequence Annotation; DNA Methylation; 3. Good health; MicroRNAs; 03 medical and health sciences; Epilepsy, Temporal Lobe; Cluster Analysis; Humans; Female

Temporal lobe epilepsy (TLE) is the most common form of adult epilepsy and frequently evolving drug resistance. Although there is growing consensus that noncoding ribonucleic acids (ncRNAs) are modulators of TLE, the knowledge about the deoxyribonucleic acid (DNA) methylation patterns of ncRNAs in TLE remains limited. In the current study, we constructed DNA methylation profiles from 30 TLE patients and 30 healthy controls for ncRNAs, primarily focusing on long ncRNAs (lncRNAs) and microRNAs (miRNAs), by reannotating data of DNA methylation BeadChip. Statistics analyses have revealed a global hypermethylation pattern in miRNA and lncRNA gene in TLE patients. Bioinformatic analyses have found aberrantly methylated miRNAs and lncRNAs are related to ion channel activity, drug metabolism, mitogen-activated protein kinase (MAPK) signaling pathway, and neurotrophin signaling pathway. Aberrantly methylated ncRNA and pathway target might be involved in TLE development and progression. The methylated and demethylated ncRNAs identified in this study provide novel insights for developing TLE biomarkers and potential therapeutic targets.