Neuroprotective Potential and Paracrine Activity of Stromal Vs. Culture-Expanded hMSC Derived from Wharton Jelly under Co-Cultured with Hippocampal Organotypic Slices

• DOI: 10.1007/s12035-017-0802-1
• Publication Date: 2017-11-13T00:20:19Z
• Authors: Sylwia Dabrowska; Joanna Sypecka; Anna Jablonska; Lukasz Strojek; Miroslaw Wielgos; Krystyna Domanska...; Anna Sarnowska
• Keywords: Wharton's jelly

Regardless of enormous translational progress in stem cell clinical application, our knowledge about biological determinants transplantation-related protection is still limited. In addition to adequate selection the source well dedicated a specific disease and optimal standardization all other pre-transplant procedures, we have decided focus more attention impact culture time environment itself on molecular properties regenerative capacity cultured vitro. The aim this investigation was determine neuroprotection-linked phenotypic functional changes that could spontaneously take place when freshly isolated Wharton's jelly mesenchymal (WJ-MSC) undergo standard selection, growth, spontaneous differentiation throughout passaging For determining their neuroprotective potential, used experimental model human WJ-MSC co-culture with intact or oxygen-glucose-deprived (OGD) rat organotypic hippocampal (OHC). It has been shown putative mechanisms mediating interactions between OHC slices relies mainly paracrine activity. Interestingly, it also found strongest protective effect exerted by umbilical cord tissue fragments first cohort cells (hMSCs) migrating out these (passage 0). Culturing WJ-derived hMSC well-controlled conditions under air atmosphere up fourth passage caused unexpected decline effectiveness toward OGD-OHC model. This further correlated substantial phenotype, profile activities as recipient reaction evaluated rat-specific gene expression.