Identifying Pyroptosis-Hub Genes and Inflammation Cell Type-Related Genes in Ischemic Stroke

• DOI: 10.1007/s12035-024-04647-x
• Publication Date: 2025-01-11T04:17:22Z
• Authors: Wei Ge; Liangbin Cao; Can Liu; Hao Wang; Meijing Lu; Yongquan Chen; Ye Wang
• Keywords: Pyroptosis

Stroke is the second-leading global cause of death. The damage attributed to immune storm triggered by ischemia–reperfusion injury (IRI) post-stroke substantial. However, data on transcriptomic dynamics pyroptosis in IRI are limited. This study aimed analyze expression key genes stroke and their correlation with infiltration. Pyroptosis-related were identified from obtained middle cerebral artery occlusion (MCAO) datasets. Differential functional analyses pyroptosis-related performed, differences enrichment between high-risk low-risk groups determined. An MCAO diagnostic model was constructed validated using selected differential expression. High- for cell analysis hub genes. A regulatory network miRNA also constructed, protein domains predicted. an rat model. Twenty-five showed expression, including four genes, namely WISP2, MELK, SDF2L1, AURKB. Characteristic verified real-time quantitative PCR analyses. high- significant SDF2L1. In infiltration analysis, 12 cells samples. Further demonstrated positive correlations gene SDF2L1 cell-activated dendritic group natural killer group. elevated closely associated inflammatory types can predict ischemic risk levels help facilitate treatment.