Melatonin Ameliorates Senescence of Mouse Auditory Cell Line HEI-OC1 Cells by Suppressing NLRP3 Inflammasome-Mediated Pyroptosis

• DOI: 10.1007/s12035-025-04880-y
• Publication Date: 2025-04-04T05:38:33Z
• Authors: Yongqing Ding; Yachao Liu; Dong Li; Ruili Hu; Zedong Tian; Lihang Yang; Yanping Li; Yantao Lin; Yan Qu
• Keywords: Pyroptosis; Senescence

The aim of this study was to determine whether oxidative stress-induced premature senescence in mouse auditory cell line HEI-OC1 cells vitro is associated with NLRP3 inflammasome activation and pyroptosis, melatonin has a protective effect. were exposed different concentrations hydrogen peroxide (H2O2) induce stress subsequently analyzed by Western blotting measure pyroptosis-related proteins - NLRP3, caspase-1, GSDMD-N. Compared untreated control cells, exposure promoted accompanied significant increase levels Furthermore, treatment shown decrease the expression these attenuate H2O2-induced process. contributes vitro, leading pyroptosis. Melatonin attenuates pyroptosis inhibiting GSDMD-N, providing reliable evidence for as potential therapeutic agent age-related hearing loss.