A cost-effective and efficient approach for generating and assembling reagents for conducting real-time PCR

• DOI: 10.1007/s12038-021-00231-w
• Publication Date: 2021-11-27T07:05:02Z
• Authors: Ridim D Mote; V Shinde Laxmikant; Surya Bansi Singh; Mahak Tiwari; Hemant Singh; Juhi Srivastava; Vidisha Tripathi; Vasudevan Seshadri; Amitabha Majumdar; Deepa Subramanyam
• Keywords: Limiting; 2019-20 coronavirus outbreak

AbstractReal-time PCR is a widely used technique for quantification of gene expression. However, commercially available kits for real-time PCR are very expensive. The ongoing coronavirus pandemic has severely hampered the economy in a number of developing countries, resulting in a reduction in available research funding. The fallout of this will result in limiting educational institutes and small enterprises from using cutting edge biological techniques such as real-time PCR. Here, we report a cost-effective approach for preparing and assembling cDNA synthesis and real-time PCR mastermixes with similar efficiencies as commercially available kits. Our results thus demonstrate an alternative to commercially available kits.