Influence of ovarian cancer type I and type II microenvironment on the phenotype and function of monocyte-derived dendritic cells

• DOI: 10.1007/s12094-017-1686-2
• Publication Date: 2017-06-06T11:36:51Z
• Authors: J. Surówka; I. Wertel; K. Okła; W. Bednarek; R. Tarkowski; J. Kotarski
• Keywords: Monocyte

The aim of this study was to evaluate the influence ovarian cancer cell lysates isolated from type I or II (OC) on phenotype monocyte-derived dendritic cells (Mo-DCs) and cytokine profile. We also determined whether Mo-DCs tumor microenvironment, reflected by peritoneal fluid (PF) cancer, could promote regulatory T (Tregs) differentiation naive CD4+ lymphocytes in vitro.Our results show a significant role microenvironment PF OC inhibition DC process. Interestingly, percentage co-expressing CD45 CD14 antigens cultures stimulated with both higher than control. Furthermore, expressing CD1a, i.e., marker immature DCs, significantly reduced OC. obtained that induce monocytes into macrophage-like CD1a+/HLA-DR+/CD83- CD86/HLA-DR expression. are able prevent an immune response release IL-10, whereas can generation Tregs.We demonstrate each unique DCs Tregs, associated immune-suppressive function, which may be obstacle while developing effective anticancer vaccination.