Construction and Analytical Application of Internal Amplification Controls (IAC) for Detection of Food Supply Chain-Relevant Viruses by Real-Time PCR-Based Assays

• DOI: 10.1007/s12161-011-9224-2
• Publication Date: 2011-04-04T04:11:47Z
• Authors: Marta Diez-Valcarce; Katarina Kovač; Nigel Cook; David Rodríguez-L...; Marta Hernández
• Keywords: Murine norovirus

Internal amplification controls (IACs) were constructed for incorporation into real-time nucleic acid assays bovine polyomavirus, hepatitis A virus, E human adenovirus, norovirus genogroup I, II, murine and porcine adenovirus. The addition of optimised amounts IAC the did not affect limits detection each specific target virus. poorly performed extraction viral acids was simulated, effectiveness IACs in identifying failed demonstrated. this study can be reliably used their to provide a robust control that routinely applied analysis foods viruses.