Assay of β-Carotene in Dietary Supplements and Fruit Juices by TLC-Densitometry

• DOI: 10.1007/s12161-014-0019-0
• Publication Date: 2014-10-16T03:58:43Z
• Authors: Małgorzata Starek; Anna Guja; Monika Dąbrowska; Jan Krzek
• Keywords: Densitometry; Ammonium hydroxide; Carrot juice; Carotene; Absorbance

β-carotene as a dietary supplement is matter of interest to anyone who seeks improve their skin condition or sight. Due its antioxidative properties, carotenoids are involved in the prevention severe conditions, such cancer, heart disease, macular degeneration, cataract, which makes them an object scientific study. Thanks color, ß-carotene used food colorant, improving appearance foodstuff. A presented method was established for identification and quantification by thin-layer chromatography with densitometric detection. As stationary phase, TLC Aluminiumoxid 60 F254 neutral utilized. It were activated methanol dried at temperature °C 1 h. The development carried out twin trough glass chamber saturated mobile phase consisting chloroform/methanol/acetone/ammonium hydroxide (10:22:53:0.2, v/v/v/v). scanner scanning analysis absorbance mode 450 nm. validated specificity, linearity, precision, accuracy, limit detection (LOD), (LOQ). Presented illustrates simple, economical, suitable manner routine quantitative qualitative determination supplements carrot juice.