Functional characterization of a biosurfactant-producing thermo-tolerant bacteria isolated from an oil reservoir

• DOI: 10.1007/s12182-011-0152-y
• Publication Date: 2011-07-15T08:04:56Z
• Authors: Jing Wang; Guang Ji; Jing Tian; Hongdan Zhang; Hanping Dong; Li Yu
• Keywords: Strain (injury); Lipopeptide

A Bacillus subtilis strain JA-1 isolated from an oil reservoir was studied. This strain is capable of growth and producing biosurfactant at a temperature of 60 °C. In nutrient medium it produced bi osurfactant which reduced the surface tension from 68.2 mN/m to 28.3 mN/m, with the critical micelle concentration (CMC) of 48 mg/L. The measured surface tension indicated that the biosurfactant possessed stable surface activity at high temperature and a specific range of pH and salt concentrations. The results of thin layer chromatography (TLC) together with FT-IR showed that the metabolic product of strain JA-1 is a lipopeptide biosurfactant. T he ability to growth at high temperature and to produce biosurfactant makes strain JA-1 promising for enhanced oil recovery.