Stress response gene activation protects sea urchin embryos exposed to X-rays

• DOI: 10.1007/s12192-011-0277-3
• Publication Date: 2011-06-30T09:07:30Z
• Authors: Rosa Bonaventura; Francesca Zito; Caterina Costa; Salvatore Giarrusso; Filippo Celi; Valeria Matranga
• Keywords: Paracentrotus lividus

We used Paracentrotus lividus sea urchin embryos, a well-established model in developmental biology and ecotoxicology, for investigation on stress/anti-apoptotic protein expression elicited response to harmful ionizing radiation, such as X-rays. evaluated the acute effects of high-dose exposure (5 Gy) P. analyzing by Western blotting accumulation levels HSP60, HSP70, BAG3 putative p63 at 24 48 h after irradiation. found an increase BAG3, only irradiation, whereas no HSP60 was detected either or h. Levels mRNA coding HSP70 were also investigated relative RT-PCR returning their initial Results demonstrate presence adaptive regulatory mechanism operating transcriptional level h, followed translational activation post-irradiation. In conclusion, our findings confirm embryo sensible bioindicator cell damage we propose this studies protective pathways activated The novel result involvement X-rays, never tested so far any other embryonic system, opens way use biomarkers X-ray hazards.