De novo assembly and characterization of Muscovy duck liver transcriptome and analysis of differentially regulated genes in response to heat stress

• DOI: 10.1007/s12192-015-0573-4
• Publication Date: 2015-02-08T00:14:46Z
• Authors: Tao Zeng; Liping Zhang; Jinjun Li; Deqian Wang; Yong Tian; Lizhi Lu
• Keywords: Sequence assembly; KEGG; Illumina dye sequencing; RNA-Seq

High temperature is a major abiotic stress limiting animal growth and productivity worldwide. The Muscovy duck (Cairina moschata), sometimes called the Barbary drake, type of with fairly unusual domestication history. In Southeast Asia, meat one top meats consumed, as such, production an important topic research. transcriptomic genomic data presently available are insufficient to understanding molecular mechanism underlying heat tolerance ducks. Thus, transcriptome expression profiling for this species required resource identifying genes developing marker. study, de novo assembly gene analysis using Illumina sequencing technology were performed. More than 225 million clean reads generated assembled into 36,903 unique transcripts average length 1,135 bp. A total 21,221 (57.50 %) unigenes annotated. Gene Ontology (GO) annotated revealed that majority sequenced associated transcription, signal transduction, apoptosis. We also performed upon treatment in ducks identified 470 heat-response transcripts. GO term enrichment showed protein folding chaperone binding significant enrichment, whereas KEGG pathway analyses Ras MAPKs activated after Our research enriched sequences information duck, provided novel insights responses these ducks, serve candidate or markers can be used guide future efforts breed heat-tolerant strains.