The synthesis of diapause-specific molecular chaperones in embryos of Artemia franciscana is determined by the quantity and location of heat shock factor 1 (Hsf1)

• DOI: 10.1007/s12192-019-00971-7
• Publication Date: 2019-01-30T08:30:29Z
• Authors: Jiabo Tan; Thomas H. MacRae
• Keywords: HSF1

The crustacean, Artemia franciscana, displays a complex life history in which embryos either arrest development and undertake diapause as cysts or they develop into swimming nauplii. Diapause entry is preceded during embryogenesis by the synthesis of specific molecular chaperones, namely small heat shock proteins p26, ArHsp21, ArHsp22, ferritin homolog, artemin. Maximal diapause-specific chaperones dependent on transcription factor, factor 1 (Hsf1), found similar amounts nauplii newly released from females. This investigation was performed to determine why, if contain comparable Hsf1, only cyst-destined synthesize chaperones. Quantification qPCR immunoprobing Western blots, respectively, demonstrated that hsf1 mRNA Hsf1 peaked day 2 post-fertilization were developing then declined. present nauplii-destined post-fertilization, but much smaller than embryos, increased quantity until release Immunofluorescent staining revealed amount nuclei greatest 4 could not be detected nauplius-destined at this time. differences location explain why fated become eventually enter artemin, whereas do produce these