Effects of Simulated Microgravity on Wild Type and Marfan hiPSCs-Derived Embryoid Bodies

• DOI: 10.1007/s12195-021-00680-1
• Publication Date: 2021-06-07T19:03:08Z
• Authors: Paola Spitalieri; Mario Marini; Maria Giovanna Sc...; Michela Murdocca; Giuliana Longo; Augusto Orlandi; Giuseppe Novelli; Federica Sangiuolo
• Keywords: Embryoid body; Regenerative Medicine; Fibrillin

Abstract Background Mechanical unloading in microgravity is thought to induce tissue degeneration by various mechanisms, including the inhibition of regenerative stem cell differentiation. In this work, we investigate effects simulation on early lineage commitment hiPSCs from healthy and Marfan Syndrome (MFS; OMIM #154700) donors, using embryoid bodies model differentiation evaluating their ultra-structural conformation. MFS involves an anomalous organization extracellular matrix for a deficit fibrillin-1, essential protein connective tissue. Methods vitro models require use derived hiPSCs. A DRPM was used simulate conditions. Results Our data suggest increase stemness those EBs maintained SMG condition. are still capable external migration, but less likely distinguish, providing measure remaining progenitor or populations earlier stage. The response appears vary between WT EBs, presumably as result structural component deficiency due fibrillin-1 lack. fact, show reduced adaptive capacity environment that prevented them reacting making rapid adjustments, while retention, without any changes Conclusion formation specifically mimics into embryonic tissues, process has also significant similarities with adult cell-based regeneration. devices maintenance cells medicine applications becoming increasingly more feasible.