Validation of Random Positioning Versus Clinorotation Using a Macrophage Model System

• DOI: 10.1007/s12217-019-9687-0
• Publication Date: 2019-03-02T00:49:08Z
• Authors: Sonja Brungs; Jens Hauslage; Ruth Hemmersbach
• Keywords: Clinostat; Mode (computer interface)

Clinostats and Random Positioning Machines (RPMs) are valuable devices for microgravity simulations in order to study fundamental gravity-dependent mechanisms on ground preparation space flights. Both have different modes of operation, which be carefully considered comprehensively discussed with respect their potential impact the quality (simulated) microgravity. Here, we used well-studied oxidative burst reaction immune cell (macrophage) model system, compare clinorotation random positioning. The RPM was a mode, rotating sample 60 rpm around horizontal axis "random speed mode" (2-10 rpm) without direction, thus, two axes either bi- or unidirectionally. production Reactive Oxygen Species (ROS) during macrophages visualized by luminescence luminol assay. During cells responded reduction ROS is fully line earlier studies (clinostat, parabolic flight). In contrast, exposure positioning showed that response RPM-exposure differs significantly from observed clinorotation, i.e. jittering, more variant form production. We can conclude our work, not suitable its real mode (with direction; 2-10 simulate conditions chosen system. recommend investigators using simulators should choose device operation specifically cellular system interest.