A Highly Pathogenic Strain of Porcine Deltacoronavirus Caused Watery Diarrhea in Newborn Piglets

• DOI: 10.1007/s12250-018-0003-8
• Publication Date: 2018-03-22T11:55:45Z
• Authors: Zhichao Xu; Huiling Zhong; Qingfeng Zhou; Yunping Du; Li Chen; Yun Zhang; Chunyi Xue; Yongchang Cao
• Keywords: Immunofluorescence

Porcine deltacoronavirus (PDCoV) is a newly identified virus that causes watery diarrhea in newborn piglets and results significant economic losses to the pig industry. Since first reported Hong Kong 2012, PDCoV has been subsequently detected USA, South Korea, Thailand, mainland China. Here we isolated strain of PDCoV, named CHN-GD-2016, from intestinal content diseased piglet with severe farm Guangdong, CHN-GD-2016 could be by immunofluorescence specific rabbit antisera, typical crown-shaped particles spiky surface projections this were observed electron microscopy. Genomic analysis showed was closely related other Chinese strains, highest sequence similarity CHN/Tianjin/2016. Importantly, inoculation 1 × 105 TCID50 oral feeding successfully reproduced clear clinical symptoms, including vomiting, dehydration, piglets. In addition, RNA rectal swabs 7 days post detected, macroscopic microscopic lesions small intestine observed, viral antigen also intestines immunohistochemical staining. Collectively, data show study confirms present China highly pathogenic