Biphasic Cholinergic Modulation of Reverberatory Activity in Neuronal Networks

• DOI: 10.1007/s12264-022-01012-7
• Publication Date: 2023-01-21T03:02:38Z
• Authors: Xiao-Wei Li; Yi Ren; Dong-Qing Shi; Lei Qi; Fang Xu; Yanyang Xiao; Pak-Ming Lau; Guo-Qiang Bi
• Keywords: Postsynaptic Current; Neuronal firing

Acetylcholine (ACh) is an important neuromodulator in various cognitive functions. However, it unclear how ACh influences neural circuit dynamics by altering cellular properties. Here, we investigated reverberatory activity cultured neuronal networks. We found that suppressed the occurrence of evoked reverberation at low to moderate doses, but a much lesser extent high doses. Moreover, doses caused longer duration reverberation, and higher spontaneous activity. With whole-cell recording from single neurons, inhibited excitatory postsynaptic currents (EPSCs) while elevating firing dose-dependent manner. Furthermore, all ACh-induced network changes were blocked muscarinic, not nicotinic receptor antagonists. computational modeling, simulated EPSCs excitability cells mimicking effects indeed modulated similar experimental observations. Thus, modulates biphasic fashion, probably inhibiting synaptic transmission facilitating through muscarinic signaling pathways.