d-ribose induces nephropathy through RAGE-dependent NF-κB inflammation

• DOI: 10.1007/s12272-018-1061-z
• Publication Date: 2018-08-13T01:57:51Z
• Authors: Jinni Hong; Xuemei Wang; Ning Zhang; Hong Fu; Weiwei Li
• Keywords: RAGE; Ribose; IκBα

Recently, aberrantly high levels of D-ribose have been discovered in type II diabetic patients. glycates proteins more rapidly than D-glucose, resulting the production advanced glycation end products (AGEs). Accumulations these can be found impaired renal function, but mechanisms are poorly understood. The present study tested whether induces dysfunction via RAGE-dependent NF-κB signaling pathway. In vivo, administration was to lower blood glucose and regulate insulin tolerance. Compared controls, urine nitrogen creatinine excretion were increased mice receiving accompanied by severe pathological damage. Furthermore, immunohistochemistry showed that NF-κB, AGEs, receptor AGEs (RAGE) kidneys with treatment. vitro, western blot immunofluorescent staining, we confirmed induced activation accumulation RAGE mesangial cells. By co-immunoprecipitation, pull-down remarkably expression NF-κB. Silencing gene blocked phosphorylation D-ribose. These results strongly suggest inflammation a manner, which may triggering mechanism leading nephropathy.