Testosterone induces activation of porcine primordial follicles in vitro

• DOI: 10.1007/s12522-010-0068-z
• Publication Date: 2010-10-14T07:05:07Z
• Authors: Manjula P. S. Mag...; Mai Zengyo; Mohammad Moniruzz...; Takashi Miyano
• Keywords: Antral follicle; Organ culture

The mechanism underlying primordial follicle activation is poorly understood. In this study, in-vitro culture and subsequent xenotransplantation were conducted to determine whether testosterone promotes the of porcine follicles.Prepubertal ovarian cortical strips containing follicles cultured in presence for 7 days, subsequently transplanted immunodeficient mice 2 months. After transplantation, development was examined histologically. androgen receptors oocytes assessed by use western blot immunohistochemical analyses.Testosterone at 10-6 m induced transition intermediate (19 ± 4%) primary (3 1%) stages after 7-day culture, while 56 5% remained initial pool. Higher concentrations, above 10-5 m, or lower below did not induce stage. with testosterone, mice. Some developed secondary (15 3%) antral (10 stages, whereas 44 7% experiment, estradiol-17β (10-7-10-5 m) had no significant effect on activation. receptor antagonist, cyproterone acetate, inhibited stimulatory activation, suggesting an receptor-mediated action testosterone. Western analyses revealed that present follicles.These results suggest vitro through oocytes.