Evaluation of Trends in Influenza A and B Viruses in Wastewater and Human Surveillance Data: Insights from the 2022–2023 Season in Italy

• DOI: 10.1007/s12560-024-09622-2
• Publication Date: 2024-12-07T13:01:10Z
• Authors: P. Mancini; D. Brandtner; C. Veneri; G. Bonanno Ferraro; M. Iaconelli; S. Puzelli; M. Facchini; G. Di Mario; P. Stefanelli; L. Lucentini; A. Muratore; ; Paolo Torlontano; Giuseppe Aprea; Silvia Scattolini; Vicdalia Aniela A...; Mariangela Mininni; Rosa Anna Cifarelli; Achille Palma; Giuseppe Lauria; Giovanna La Vecchia; Edoardo Malacaria; Giuseppe Folino; Adelaide Calabria; Giorgia Bulotta; Angelo D’Argenzio; Luigi Cossentino; Giovanna Fusco; Maurizio Viscardi; Alessandra Tosco; Amalia Porta; Antonia Voli; Francesca Pennino; Annalisa Lombardi; Paola Angelini; Daniele Nasci; Giovanni Alborali; Nicoletta Formenti; Flavia Guarneri; Nadia Fontani; Marco Guercio; Lisa Gentili; Marika Mariuz; Gabriella Trani; Anna Pariani; Laura De Lellis; Carla Ancona; Alessandra Barca; Flavia Serio; Doriana Antonella...; Irene Ferrante; Monica Monfrinotti; Maria Teresa Scic...; Antonella Cersini; Gabriele Pietrella; Claudio Ottaviano; Mariaconcetta Arizzi; Acea Elabori; Elena Nicosia; Nadia Fontani; Marco Guercio; Elena Grasselli; Alberto Izzotti; Irene Tomesani; Marta Bellisomi; Stefano Rosatto; Emanuela Ammoni; Danilo Cereda; Barbara Bertasi; Desdemona Oliva; Maria Giovanna Guiso; Sara Castiglioni; Silvia Schiarea; Sandro Binda; Valeria Primache; Laura Pellegrinelli; Clementina Cocuzza; Andrea Franzetti; Luigi Bolognini; Fabio Filippetti; Marta Paniccia’; Sara Briscolini; Silvia Magi; Annalisa Grucci; Michele Colitti; Angela Ciccaglione; Carmen Montanaro; Giuseppe Aprea; Silvia Scattolini; Vicdalia Aniela A...; Bartolomeo Griglio; Angela Costa; Lucia Decastelli; Angelo Romano Man...; Elisabetta Carraro; Cristina Pignata; Lisa Richiardi; Silvia Bonetta; Nehludoff Albano; Giuseppe Di Vittorio; Onofrio Mongelli; Francesca Apollonio; Francesco Triggiano; Osvalda De Giglio; Maria Teresa Mont...; Mario Palermo; Carmelo Massimo M...; Walter Mazzucco; Fabio Tramuto; Simona De Grazia; Giovanni Maurizio...; Chiara Filizzolo; Giuseppa Purpari; Francesca Gucciardi; Margherita Ferrante; Antonella Agodi; Martina Barchitta; Piergiuseppe Cala; Annalaura Carducci; Marco Verani; Ileana Federigi; Matteo Ramazzotti; Gian Maria Rossolini; Salvatore Macrì; Ermanno Federici; Maya Petricciuolo; Agnese Carnevali; Mauro Ruffier; Lorena Masieri; Eric Grange; Florida Damasco; Francesca Russo; Gisella Pitter; Vanessa Groppi; Franco Rigoli; Marco Zampini; Tatjana Baldovin; Irene Amoruso; Maria Cadonna; Mattia Postinghel; Paola Foladori; Francesca Cutrupi; Alberta Stenico; Demetz Lea; Morelli Marco; Dossena Matteo; E. Suffredini; G. La Rosa
• Keywords: Surveillance; dPCR; Research; 610; influenza virus; surveillance; wastewater; dPCR; Wastewater; Influenza virus; Influenza virus; Surveillance; Wastewater; dPCR; Influenza viru; Influenza virus, Wastewater, dPCR, Surveillance

Wastewater-based epidemiology (WBE) is a recognized, dynamic approach to monitoring the transmission of pathogens in communities through urban wastewater. This study aimed detect and quantify influenza A B viruses Italian wastewater during 2022–2023 season (October 2022 April 2023). total 298 samples were collected from 67 treatment plants (WTPs) across country. These analyzed for (IAV, IBV) using primers originally developed by Centers Disease Control Prevention (CDC) real-time PCR adapted digital PCR. The overall detection rates IAV IBV entire period 19.1% 16.8%, respectively. prevalence showed gradual increase October December 2022, peaking at 61% December. In contrast, peaked 36% February 2023. temporal discrepancy peak concentrations suggests different seasonal patterns two types. trends mirrored human surveillance data, which cases 46% late declining around 2% 2023, starting significantly January 2023 about 14% March. ranged 9.80 × 102 1.94 105 g.c./L, while 1.07 103 1.43 104 g.c./L. Overall, environmental data consistent with observed results demonstrate value WBE tracking epidemiological highlight its potential as complementary tool infectious diseases systems.