Random laser with whole blood as scattering source

• DOI: 10.1007/s12596-025-02469-1
• Publication Date: 2025-03-03T07:27:43Z
• Authors: E. Mendicuti; C. García-Segundo; M. Villagrán-Muniz; Ricardo U. Macías...

Abstract There is a growing interest in the field of random laser emission, for incorporating biological material as an intrinsic component of the active media, in view of the multiple applications for analysis and sensor of targeted features. Here, we introduce an experimental methodology and related experimental results showing the generation of random laser emission from an active media made of Rhodamine 6G (R6G) in liquid solution with whole blood cells (WBC) as scattering agent. In a descriptive manner, we report how to combine solvent conditions and concentrations of WBC and alongside Rhodamine 6G, for successfully inducing the random laser action. The observation of the response for the different experimental conditions we describe, is made through spectroscopic analysis. As part of the methodology, in a self-comparative manner the presence and absence of the random laser action is shown to be related to the WBC light scattering of the R6G fluorescence emission. In addition to achieving the known random laser emission at ~ 567 nm, generally obtained with neutral particles, now obtained using WBC as particles of the colloid, an additional band centered at ~ 596 nm of wavelength is simultaneously visible; and also capable of producing random laser emission. As a figure of merit we display life-time evolution of both emission lines, their gain-like curves, as function of energy and for different concentrations of WBC. The novelty, in addition to getting the conditions for inducing the random laser action using WBC as a scatterer, is that for this colloid we get the additional random laser emission at ~ 596 nm, with R6G as fluorescent agent. Therefore, out of this experimental method, we show how to place feasible conditions for the optical scattering from whole blood to perform as the intrinsic stochastic component of the random laser system.