Studies of the Impact of the Bifidobacterium Species on Inducible Nitric Oxide Synthase Expression and Nitric Oxide Production in Murine Macrophages of the BMDM Cell Line

• DOI: 10.1007/s12602-023-10093-3
• Publication Date: 2023-05-25T11:02:25Z
• Authors: Agnieszka Zabłocka; Dominika Jakubczyk; Katarzyna Leszczy...; Katarzyna Pacyga-...; Józefa Macała; Sabina Górska
• Keywords: Bifidobacterium animalis; Bifidobacterium longum; Bifidobacterium breve

Abstract Bifidobacterium species are one of the most important probiotic microorganisms which present in both, infants and adults. Nowadays, growing data describing their healthy properties arise, indicating they could act at cellular molecular level. However, still little is known about specific mechanisms promoting beneficial effects. Nitric oxide (NO), produced by inducible nitric synthase (iNOS), involved protective gastrointestinal tract, where it can be provided epithelial cells, macrophages, or bacteria. The study explored whether induction iNOS-dependent NO synthesis macrophages stems from action species. ability ten strains belonging to 3 different ( longum , adolescentis animalis ) activate MAP kinases, NF-κB factor, iNOS expression a murine bone-marrow-derived cell line was determined Western blotting. Changes production were Griess reaction. It performed that able induce NF-қB-dependent production; however, efficacy depends on strain. highest stimulatory activity observed for subsp. animals CCDM 366, whereas lowest noted 371 372. Both TLR2 TLR4 receptors -induced macrophage activation production. We showed impact regulation MAPK kinase activity. Using pharmaceutical inhibitors ERK 1/2 JNK, we confirmed these kinases control mRNA expression. Concluding, may mechanism intestine, strain-dependent.