Estimation of Uncertainty in the Determination of Serum Electrolytes (Na, K, Ca, Mg) by Flame Atomic Absorption Spectroscopy

• DOI: 10.1007/s12647-017-0244-2
• Publication Date: 2018-01-08T06:51:25Z
• Authors: A. M. García-Alegría; M. G. Cáñez-Carrasco; M. Serna-Félix; K. K. Encinas Soto; A. Gómez-Álvarez
• Keywords: 01 natural sciences; 0104 chemical sciences; 3. Good health

From a standardized and validated method to quantify serum electrolytes (Na, K, Ca and Mg) by flame atomic absorption spectroscopy, combined relative uncertainty (CRSU) and the percentage of combined relative standard uncertainty (% CRSU) were determined. For this, analytical grade standards were used and later from a certified reference material (NIST2670a). Previously, the sources of uncertainty used in the measurement using the Ishikawa diagram (cause-effect) were established, including mass concentration, calibration curves, volume, reactive targets, certified reference material, dilution and repeatability. Quantification of the metals was performed using the atomic absorption spectroscopy technique employing a Perkin Elmer model 3100 equipment. The values obtained for the CRSU on average are below 0.030 and for the % CRSU on average they are below 3.1%. These results indicate that the uncertainty values are acceptable since they are below the recommended values for these metals.