Modified Recording Chamber for Sustained Kainate-Induced Gamma Oscillations in Submerged Rat Hippocampal Slices

• DOI: 10.1007/s12668-016-0313-y
• Publication Date: 2016-10-10T21:39:08Z
• Authors: Elvira Juzekaeva; Azat Nasretdinov; Marat Mukhtarov
• Keywords: Kainate receptor

© 2016, Springer Science+Business Media New York.Fast neuronal network oscillations in an in vitro model are a challenging task. Here, we report that sustained gamma (31–35 Hz) oscillations can be induced by kainate (50 nM) in submerged rat hippocampal slices using modified Hájos’s type recording chamber with a superfusion inlet positioned close to the CA3 pyramidal cell layer. The general features of these kainate-induced gamma oscillations were similar to those previously reported in the hippocampal slices using the interface-type chamber and superfused hippocampus in vivo. We suggest that close positioning of the superfusion inlet improves oxygen supply and temperature control of the oscillation-generating network and that this modification could be useful in studies of the gamma rhythmogenesis in the submerged slices.