The ratio of CD8 + lymphocytes to tumor-infiltrating suppressive FOXP3 + effector regulatory T cells is associated with treatment response in invasive breast cancer

• DOI: 10.1007/s12672-022-00482-5
• Publication Date: 2022-04-19T11:03:33Z
• Authors: Noriko Goda; Shinsuke Sasada; Hideo Shigematsu; Norio Masumoto; Koji Arihiro; Hiroyoshi Nishikawa; Shimon Sakaguchi; Morihito Okada; Takayuki Kadoya
• Keywords: Tumor-infiltrating lymphocytes

FOXP3 + and CD8 are recognized markers of tumor-infiltrating lymphocytes (TILs) for breast cancer. TILs composed effector Tregs (eTregs) other subpopulations that classified by their differences in suppressive function. In this prospective study, we evaluated Treg cancer.84 patients with cancer were enrolled. Fresh extracted andTregs into eTregs (CD4+FOXP3highCD45RA-), FOXP3+ subsets (naïve non-Tregs), total CD8+CD4- using flow cytometry. The suppression strength each subpopulation was analyzed. association between TIL subpopulations, clinicopathological characteristics, response to chemotherapy evaluated.The mean CD8/eTreg ratio value 7.86 (interquartile range: 4.08-12.80). proliferation function significantly suppressed compared the (proliferation rates: control: 89.3%, naiiveTreg: 64.2%, non-Treg: 78.2% vs eTreg 1.93%; all P < 0.05). high a /eTreg achieved excellent pathological complete (pCR) rate neoadjuvant (90.2%) independent predictive factors pCR (odds ratio:18.7(confidence interval 1.25-279) A detailed assessment patient who underwent NAC revealed showed higher low CD8/FOXP3 (39.6% 13.3, 0.05) triple negative subtype stromal 50%.A enhances invasive