Characterization of 28 polymorphic SSR loci from Meretrix lamarkii Deshayes

• DOI: 10.1007/s12686-013-0032-4
• Publication Date: 2013-08-29T12:07:04Z
• Authors: Bo Liu; Shuang-shuang Teng; Xue-Liang Chai; Yan-Qing Shao; Guo-Qiang Xiao; Jun Fang; Jiong-ming Zhang
• Keywords: Linkage Disequilibrium; Bonferroni correction

In this study, 28 polymorphic simple sequence repeats (SSR) markers were isolated from an expressed sequence tag (EST) library of Meretrix lamarkii Deshayes. The number of alleles of these 28 EST-SSR markers varied from 2 to 8, with a mean of 3.64 alleles per locus. The observed, expected heterozygosity and polymorphic information content per locus varied from 0.10 to 1.00, 0.10 to 0.86 and 0.09 to 0.81, respectively. No significant linkage disequilibrium between pairs of loci was found, except two of them significantly deviated from Hardy–Weinberg equilibrium after Bonferroni correction. These loci are the first time to be characterized and will be useful for population genetic structure investigation and marker assisted breeding for M. lamarkii.