Role of phosphoglucomutase in regulating trehalose metabolism in Nilaparvata lugens

• DOI: 10.1007/s13205-020-2053-5
• Publication Date: 2020-01-23T07:03:08Z
• Authors: Bi-Ying Pan; Yong-Kang Liu; Hong-Kai Wu; Xiao-Qing Pang; Shi-Gui Wang; Bin Tang; Cai-Di Xu
• Keywords: Phosphoglucomutase

Abstract Phosphoglucomutase (PGM) is a key enzyme in glycolysis and gluconeogenesis, regulating both glycogen trehalose metabolism insects. In this study, we explored the potential function of phosphoglucomutase using RNA interference technology Nilaparvata lugens , brown planthopper. PGM1 PGM2 were found highly expressed midgut planthoppers, with different expression levels instar nymphs. The glycogen, glucose, also significantly increased after planthoppers injected dsRNA targeting (dsPGM1) or (dsPGM2). addition, injection dsPGM1 dsPGM2 resulted membrane-bound trehalase activity but not soluble activity. Furthermore, genes related to decreased dsPGM2. involved chitin planthopper insects showed wing deformities difficulty molting following RNAi. We suggest that silencing directly inhibits metabolism, leading impaired synthesis.