Detection of viable but non cultivable Escherichia coli after UV irradiation using a lytic Qβ phage

• DOI: 10.1007/s13213-010-0017-4
• Publication Date: 2010-02-05T16:32:33Z
• Authors: Myriam Ben Said; Otaki Masahiro; Abdennaceur Hassen
• Keywords: Lytic cycle

In order to qualify the germicidal efficacy of ultraviolet (UV) disinfection system, we generally determine reduction viable bacteria after UV-C irradiation. However, simple count and cultivable in usual media cannot reflect whether or not UV dose applied disinfect water is sufficient inactivate bacteria. Indeed, there a bacterial mix UV-treated water: dead bacteria, but noncultivable (VBNC). The third type can constitute potential risk for public health. fact, VBNC be active cause diseases. Consequently, combination conventional method used measure colony-forming ability determination adsorption constants lytic Qβ phage relation irradiated host cells by an increased (Escherichia coli ATCC 13965) allows detection which lose their cultivability growth media, keep susceptibility.