Cellulase and xylanase production by Aspergillus species

• DOI: 10.1007/s13213-011-0214-9
• Publication Date: 2011-02-05T14:17:34Z
• Authors: Marilia Ribeiro S...; Rosemery Batista ...; Milena Fernandes ...; Gorete Ribeiro de...; Ana Lúcia Figueir...
• Keywords: 0106 biological sciences; 01 natural sciences

This study aimed to produce cellulases and xylanase from Aspergillus species in submerged cultivation using sugarcane bagasse as substrate. Nine strains of Aspergillus were obtained from the Colecao de Culturas Micoteca [University Recife Mycology (URM)] and were cultivated in medium containing raw and processed sugarcane bagasse (RSB and PSC, respectively) for 5 days at 30°C and 90 rpm. The production of enzymes was evaluated by determining the activity of total cellulase, endoglucanase, exoglucanase, cellobiase, and xylanase. RSB was the best inducer for total cellulase and xylanase whereas PSB was best for exoglucanase. The production of endoglucanase and cellobiase at higher levels was independent of the type of sugarcane bagasse used. The strains that produced cellulases and xylanase at the highest levels were A. phoenicis URM 4924 and A. aculeatus URM 4953 using RSB and A. carbonarius URM 1546 using PSB medium.